The Hillman Live Cell Imaging Facility provides a unique methodology and instrumentation that allows the study of the DNA damage response in live cells in 3D and in real time. This state-of-the-art system couples an IX81/FV-1000 Olympus filter-based confocal microscopy system for high resolution imaging analysis with a 50 mW, 405 nM damage-inducing laser, which can induce DNA damage restricted to one cell nucleus within a width of less than 1 μm.
The system induces localized oxidative DNA damage including DNA base modifications, DNA single-strand breaks, and double-strand breaks within a single cell. This capability is unique in comparison to other confocal laser microscopy systems, and is particularly valuable for probing how target proteins respond to DNA damage.
Li Lan, M.D., Ph.D.